Davis J M, Egelkrout E E, Coleman G D, Chen T H, Haissig B E, Riemenschneider D E, Gordon M P
School of Forest Resources and Conservation, University of Florida, Gainesville 32611.
Plant Mol Biol. 1993 Oct;23(1):135-43. doi: 10.1007/BF00021426.
Two wound-inducible cDNAs from poplar leaves show sequence identity to vegetative storage proteins (VSP) that accumulate seasonally in poplar bark tissues. We have compared the genomic organization, cDNA sequences and expression of the genes encoding the wound-inducible cDNAs (win4) with that of a bark VSP (called bark storage protein, or BSP). There appear to be several win4 genes in the poplar genome which segregate as a single locus and are therefore likely to be clustered. The same is true of the BSP genes. The win4 locus is linked (map distance of 5 cM) to the BSP locus, consistent with a common evolutionary origin of the genes. A near full-length win4 cDNA shows 75% sequence identity to BSP cDNAs. Both win4 and BSP are systemically wound-inducible; win4 transcripts accumulate in leaves and stems, whereas BSP transcripts accumulate almost exclusively in stems. A phloem transport-dependent signaling mechanism appears to be involved in systemic win4 expression after wounding. In contrast to BSP gene expression, win4 genes are not expressed in response to short day conditions. The data indicate win4 and BSP genes are differentially regulated, and their products may play important roles in the storage and reallocation of nitrogen in perennial plants.
从杨树叶片中分离出的两个创伤诱导型cDNA与杨树树皮组织中季节性积累的营养贮藏蛋白（VSP）具有序列同源性。我们比较了编码创伤诱导型cDNA（win4）的基因与一种树皮VSP（称为树皮贮藏蛋白，或BSP）的基因组结构、cDNA序列和表达情况。杨树基因组中似乎有几个win4基因，它们作为一个单一位点分离，因此可能是成簇的。BSP基因也是如此。win4位点与BSP位点连锁（图距为5 cM），这与这些基因的共同进化起源一致。一个近乎全长的win4 cDNA与BSP cDNA的序列同源性为75%。win4和BSP都可被系统地创伤诱导；win4转录本在叶片和茎中积累，而BSP转录本几乎只在茎中积累。创伤后，韧皮部运输依赖的信号传导机制似乎参与了win4的系统表达。与BSP基因表达不同，win4基因在短日照条件下不表达。这些数据表明win4和BSP基因受到不同的调控，它们的产物可能在多年生植物氮的贮藏和重新分配中发挥重要作用。
