鉴定10-甲酰四氢叶酸、四氢叶酸和5-甲基四氢叶酸为大鼠胆汁中主要的还原型叶酸衍生物。

Identification of 10-formyltetrahydrofolate, tetrahydrofolate and 5-methyltetrahydrofolate as major reduced folate derivatives in rat bile.

作者信息

Shin H C, Shimoda M, Kokue E, Takahashi Y

机构信息

Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.

出版信息

J Chromatogr. 1993 Oct 22;620(1):39-46. doi: 10.1016/0378-4347(93)80049-a.

DOI:10.1016/0378-4347(93)80049-a

PMID:8106590

Abstract

Reduced folate derivatives in rat bile were examined using high-performance liquid chromatography with electrochemical detection (HPLC-ED). Three peaks of folate compounds were observed on the chromatograms. From the retention-time profiles and hydrodynamic voltammograms, and the profiles of ultraviolet (UV) absorbance spectra obtained by HPLC with photodiode array detection, these 3 peaks were identified as 10-formyltetrahydrofolate (10-HCO-H4PteGlu), tetrahydrofolate (H4PteGlu) and 5-methyltetrahydrofolate (5-CH3-H4PteGlu). The rates of bile secretion of 10-HCO-H4PteGlu, H4PteGlu and 5-CH3H4PteGlu were 314 +/- 181, 321 +/- 179 and 449 +/- 198 ng/h (mean +/- S.D.), respectively. 10-HCO-H4PteGlu and H4PteGlu together wtih 5-CH3-H4-PteGlu are found to be the major folate derivatives in rat bile. The nonmethylated folates, 10-HCO-H4PteGlu and H4PteGlu, may also play an important role in folate homeostasis.

摘要

采用高效液相色谱-电化学检测法（HPLC-ED）对大鼠胆汁中的还原型叶酸衍生物进行了检测。在色谱图上观察到了三个叶酸化合物峰。根据保留时间谱、流体动力伏安图以及通过配备光电二极管阵列检测的HPLC获得的紫外（UV）吸收光谱图，这三个峰分别被鉴定为10-甲酰基四氢叶酸（10-HCO-H4PteGlu）、四氢叶酸（H4PteGlu）和5-甲基四氢叶酸（5-CH3-H4PteGlu）。10-HCO-H4PteGlu、H4PteGlu和5-CH3-H4PteGlu的胆汁分泌速率分别为314±181、321±179和449±198 ng/h（平均值±标准差）。发现10-HCO-H4PteGlu、H4PteGlu与5-CH3-H4-PteGlu共同构成大鼠胆汁中的主要叶酸衍生物。未甲基化的叶酸，即10-HCO-H4PteGlu和H4PteGlu，可能在叶酸稳态中也起着重要作用。