Sundstrom P, Aliaga G R
Department of Microbiology, University of North Texas Health Science Center at Fort Worth.
J Infect Dis. 1994 Feb;169(2):452-6. doi: 10.1093/infdis/169.2.452.
Immunoblot analysis showed that enolase is one of a subset of proteins found in cell supernatants of Candida albicans. Enzyme assays on whole cell extracts indicated that enolase is an abundant protein, comprising 0.7% and 2.0% of the total protein from yeast and hyphal forms of C. albicans, respectively. Comparison of enolase enzyme activities in whole cell extracts and cell culture supernatants showed the enzyme to be located primarily within cells. Extracellular glyceraldehyde-3-phosphate dehydrogenase activity was absent or lower than that of enolase, despite equivalent intracellular levels. The results suggest that enolase, released from fungi in the absence of host factors, may contribute to enolase found circulating in the blood of patients with hematogenously disseminated candidiasis. In addition, the release from cells of highly immunogenic fungal proteins, such as enolase, may be important in defining the selective stimulation of host antifungal responses during infection.
免疫印迹分析表明，烯醇化酶是白色念珠菌细胞上清液中发现的一组蛋白质之一。对全细胞提取物的酶活性测定表明，烯醇化酶是一种丰富的蛋白质，分别占白色念珠菌酵母型和菌丝型总蛋白的0.7%和2.0%。全细胞提取物和细胞培养上清液中烯醇化酶活性的比较表明，该酶主要位于细胞内。尽管细胞内水平相当，但细胞外甘油醛-3-磷酸脱氢酶活性不存在或低于烯醇化酶。结果表明，在没有宿主因子的情况下从真菌释放的烯醇化酶可能导致在血行播散性念珠菌病患者血液中循环的烯醇化酶。此外，从细胞中释放高免疫原性真菌蛋白，如烯醇化酶，可能在确定感染期间宿主抗真菌反应的选择性刺激方面很重要。
