Milasin J, Pujić N, Dedović N, Gavrić M, Vranić V, Petrović V, Minić A
Institute of Biology and Human Genetics, School of Stomatology, Belgrade, Yugoslavia.
Int J Oral Maxillofac Surg. 1993 Dec;22(6):359-61. doi: 10.1016/s0901-5027(05)80668-x.
The DNA from 17 specimens of pleomorphic adenoma of the salivary glands was screened for the presence of ras gene mutations, which are known to be involved in the pathogenesis of various human neoplasias. By a sensitive method of hybridization with synthetic oligonucleotide probes on in vitro amplified tumor DNA, point mutations, mainly in codon 12 of the H-ras gene, were detected in six tumor specimens (35%). This high incidence of mutated ras genes suggests that their alteration may play a role in the pathogenesis of pleomorphic adenomas.
对17例涎腺多形性腺瘤标本的DNA进行筛查，以检测是否存在ras基因突变，已知该突变与多种人类肿瘤的发病机制有关。通过一种在体外扩增的肿瘤DNA上与合成寡核苷酸探针杂交的灵敏方法，在6例肿瘤标本（35%）中检测到点突变，主要发生在H-ras基因的第12密码子。ras基因突变的高发生率表明，其改变可能在多形性腺瘤的发病机制中起作用。
