Stern M, Dodson T B, Longaker M T, Lorenz H P, Harrison M R, Kaban L B
Department of Oral and Maxillofacial Surgery, University of California, San Francisco.
Int J Oral Maxillofac Surg. 1993 Dec;22(6):371-4. doi: 10.1016/s0901-5027(05)80672-1.
Sequential surgical procedures in cleft lip/palate (CL/P) patients result in scar formation that is believed to be associated with midface growth retardation. By use of a previously developed fetal lamb model, wound healing characteristics were investigated after in utero CL repair. It is hypothesized that scarless healing after fetal CL repair occurs without inflammation and scar formation. CL wounds were created in mid-second-trimester fetal lambs and either repaired in three layers (mucosa, muscle, and skin) or left unrepaired. Fetuses were then harvested at 7, 14, and 21 days postoperatively, and the wound site was examined microscopically. When created at 75 days' gestation (term = 145 days), fetal lamb CL wounds heal rapidly without inflammation and scar formation. By day 21 postoperatively, there was complete regeneration of skin, muscle, and mucosa, as well as epidermal appendages. With this model, it will be possible to compare the effects of scarless fetal CL repair with those of postnatal repair on midface growth.
唇腭裂（CL/P）患者的序贯性外科手术会导致瘢痕形成，而这种瘢痕被认为与面中部生长发育迟缓有关。通过使用先前建立的胎羊模型，对子宫内唇裂修复后的伤口愈合特征进行了研究。研究假设是，胎儿唇裂修复后可实现无瘢痕愈合，且不会出现炎症和瘢痕形成。在妊娠中期的胎羊身上制造唇裂伤口，然后将其分三层（黏膜、肌肉和皮肤）进行修复或不进行修复。随后在术后7天、14天和21天采集胎儿，对伤口部位进行显微镜检查。当在妊娠75天（足月为145天）时制造唇裂伤口，胎羊唇裂伤口可迅速愈合，且不会出现炎症和瘢痕形成。术后21天时，皮肤、肌肉和黏膜以及表皮附属器实现了完全再生。利用该模型，将有可能比较胎儿唇裂无瘢痕修复与出生后修复对面中部生长的影响。
