Hernandez F, Rivera P, Tozawa H, Hosaka Y
Department of Preventive Medicine, Osaka University, Japan.
J Electron Microsc (Tokyo). 1993 Oct;42(5):346-50.
Two glycoproteins (HN and F) of parainfluenza virus were immunogold-labeled with polyclonal and monoclonal antibodies, respectively, and their labeling patterns were compared. Both glycoproteins HN and F were efficiently and homogeneously labeled with polyclonal antibodies, whereas they were labeled much less and heterogeneously with monoclonal antibodies. When either protein was initially labeled with monoclonal antibody, and then the other one, with polyclonal antibody, immunolabels of two glycoproteins were almost completely segregated. Although this segregation deformed virion morphology, it supported the concept of monoclonal antibody-mediated movements of glycoproteins.
分别用多克隆抗体和单克隆抗体对副流感病毒的两种糖蛋白（HN和F）进行免疫金标记，并比较它们的标记模式。糖蛋白HN和F均被多克隆抗体高效且均匀地标记，而被单克隆抗体标记的程度则低得多且不均匀。当先用单克隆抗体标记其中一种蛋白，然后用多克隆抗体标记另一种蛋白时，两种糖蛋白的免疫标记几乎完全分离。尽管这种分离使病毒粒子形态变形，但它支持了单克隆抗体介导糖蛋白运动的概念。
