Ray R, Compans R W
J Gen Virol. 1987 Feb;68 ( Pt 2):409-18. doi: 10.1099/0022-1317-68-2-409.
Monoclonal antibodies to the envelope glycoproteins, HN and F, of human parainfluenza virus type 3 were coupled to a Sepharose 4B matrix and used for affinity purification of the viral glycoproteins. The purity of the glycoproteins was demonstrated by SDS-PAGE followed by fluorography or silver staining. The antigenicity of the glycoproteins was determined by immunization of rabbits; polyclonal rabbit antisera demonstrated inhibition of functional activities of the virus glycoproteins. The F glycoprotein, when reconstituted into lipid vesicles, showed distinct spike-like projections similar to those of intact virions.
针对人副流感病毒3型包膜糖蛋白HN和F的单克隆抗体与琼脂糖4B基质偶联，用于病毒糖蛋白的亲和纯化。通过SDS-PAGE随后进行荧光自显影或银染来证明糖蛋白的纯度。通过兔免疫来确定糖蛋白的抗原性；兔多克隆抗血清显示出对病毒糖蛋白功能活性的抑制作用。当F糖蛋白重组到脂质小泡中时，显示出与完整病毒粒子相似的明显的刺状突起。
