Castex F, Jouvert S, Bastide M, Corthier G
Unité de Recherche en Immunologie, Faculté de Pharmacie, Université de Montpellier 1, France.
J Med Microbiol. 1994 Feb;40(2):102-9. doi: 10.1099/00222615-40-2-102.
The kinetics of the appearance of intestinal lesions induced by orogastric inoculation of gnotobiotic mice with a lethal strain of Clostridium difficile (VPI) that produced toxins A and B in vivo and in vitro was studied and compared with the lesions induced by non-lethal C. difficile strain 786 that produced toxins A and B in vitro but only toxin B in measurable amounts in vivo. Different portions of the intestine were removed 12, 20, 26 and 30 h after inoculation and studied by scanning electronmicroscopy. The remaining portions were homogenised for enumeration of C. difficile and quantification of toxin A by enzyme immunoassay and toxin B by cytotoxicity. The results showed that, following inoculation: (i) measurable amounts of both toxins were necessary to produce lesions; (ii) with strain VPI, the caecum and the colon were rapidly impaired and completely destroyed after 1 day, whereas the small intestine was damaged to a lesser extent; (iii) C. difficile strain 786 did not cause mucosal damage but induced mucus-like or serum-like secretion and morphological changes in the caecal enterocytes only.
研究了无菌小鼠经口胃接种在体内和体外均产生毒素A和B的致死性艰难梭菌（VPI）菌株后肠道病变出现的动力学，并与在体外产生毒素A和B但在体内仅产生可测量量毒素B的非致死性艰难梭菌786菌株所诱导的病变进行了比较。接种后12、20、26和30小时，取出肠道的不同部分，通过扫描电子显微镜进行研究。其余部分进行匀浆，用于艰难梭菌计数以及通过酶免疫测定法定量毒素A，并通过细胞毒性法定量毒素B。结果表明，接种后：（i）产生病变需要可测量量的两种毒素；（ii）对于VPI菌株，盲肠和结肠在1天后迅速受损并完全破坏，而小肠受损程度较小；（iii）艰难梭菌786菌株未引起粘膜损伤，但仅诱导盲肠肠细胞出现粘液样或血清样分泌以及形态学变化。
