Castex F, Corthier G, Jouvert S, Elmer G W, Lucas F, Bastide M
Unité de Recherche en Immunologie, Faculté de Pharmacie, Université de Montpellier I, France.
J Gen Microbiol. 1990 Jun;136(6):1085-9. doi: 10.1099/00221287-136-6-1085.
The ability of Saccharomyces boulardii to protect mice against intestinal pathology caused by toxinogenic Clostridium difficile was studied. Different regions of the intestine of experimental mice were prepared for observation by scanning electron microscopy or homogenized for C. difficile enumeration and quantification of toxin A by enzyme immunoassay and toxin B by cytotoxicity. The test group was treated for 6 d with an S. boulardii suspension in drinking water and challenged with C. difficule on day 4. The three control groups were: axenic mice, mice treated with only S. boulardii and mice only challenged with C. difficile. The results showed that: (i) 70% of the mice infected by C. difficile survived when treated with S. boulardii; (ii) the C. difficile-induced lesions on the small and large intestinal mucosa were absent or markedly less severe in S. boulardii-treated mice; and (iii) there was no decrease in the number of C. difficile but rather a reduction in the amount of toxins A and B in S. boulardii-treated mice.
研究了布拉氏酵母菌保护小鼠免受产毒素艰难梭菌引起的肠道病变的能力。对实验小鼠肠道的不同区域进行处理，以便通过扫描电子显微镜观察，或进行匀浆以对艰难梭菌进行计数，并通过酶免疫测定法定量毒素A，通过细胞毒性法定量毒素B。试验组用饮用水中的布拉氏酵母菌悬液处理6天，并在第4天用艰难梭菌攻击。三个对照组分别为：无菌小鼠、仅用布拉氏酵母菌处理的小鼠和仅用艰难梭菌攻击的小鼠。结果表明：（i）用布拉氏酵母菌处理时，70%感染艰难梭菌的小鼠存活；（ii）在经布拉氏酵母菌处理的小鼠中，艰难梭菌诱导的小肠和大肠黏膜病变不存在或明显较轻；（iii）在经布拉氏酵母菌处理的小鼠中，艰难梭菌数量没有减少，而是毒素A和B的量有所减少。
