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卵形芽孢八叠球菌中一种类咕啉蛋白的结晶及初步X射线衍射研究。

Crystallization and preliminary X-ray diffraction studies of a corrinoid protein from Sporomusa ovata.

作者信息

Wagner U G, Stupperich E, Aulkemeyer P, Kratky C

机构信息

Institut für Physikalische Chemie, Karl-Franzes-Universität Graz, Austria.

出版信息

J Mol Biol. 1994 Feb 11;236(1):388-9. doi: 10.1006/jmbi.1994.1148.

DOI:10.1006/jmbi.1994.1148
PMID:8107124
Abstract

Crystals of a 40 kDa p-cresolyl-cobamide containing protein from Sporomusa ovata have been obtained from polyethyleneglycol solutions at pH 8.5 by the hanging drop technique. The crystals belong to space group C222(1) with cell dimensions a = 110.5(0.2) A, b = 144.0 (0.2) A, c = 110.4 (0.1) A. They diffract to 2.2 A resolution on a rotating anode X-ray source and are suitable for high resolution X-ray diffraction studies.

摘要

通过悬滴法从pH 8.5的聚乙二醇溶液中获得了来自卵形芽孢杆菌的一种含40 kDa对甲酚基钴胺素的蛋白质晶体。这些晶体属于空间群C222(1),晶胞参数为a = 110.5(0.2) Å,b = 144.0 (0.2) Å,c = 110.4 (0.1) Å。它们在旋转阳极X射线源上衍射至2.2 Å分辨率,适用于高分辨率X射线衍射研究。

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