Trigg P I, Shakespeare P G, Burt S J, Kyd S I
Parasitology. 1975 Oct;71(2):199-209. doi: 10.1017/s0031182000046655.
RNA extracted from purified parasites of Plasmodium knowlesi was fractionated using agarose gel electrophoresis. Preparations from para sites grown both in vivo and in vitro contained species of RNA with sedimentation coefficients of 4-0S, 5-0S, 16-6S, 24-2S, 31-4S, 38-0S and 48-3S. There was less RNA present in parasites grown in vitro than the equivalent stage parasites grown in vivo but the proportional amounts of the various species of RNA was similar in both cases. It is suggested that the 24-2S and 16-6S species of RNA are ribosomal and that the high molecular weight 31-4S, 38-0S and 48-0S species are ribosomal precursors. Ribosomal RNA synthesis occurs throughout the cell cycle during growth from the ring to the schizont stage; maximum incorporation of [H3]-adenosine occurs at the late trophozoite stage before nuclear division.
从诺氏疟原虫纯化的寄生虫中提取的RNA，用琼脂糖凝胶电泳进行分级分离。体内和体外培养的寄生虫制备物中均含有沉降系数为4.0S、5.0S、16.6S、24.2S、31.4S、38.0S和48.3S的RNA种类。体外培养的寄生虫中的RNA比体内培养的同等阶段寄生虫中的RNA少，但两种情况下各种RNA种类的比例相似。有人认为，24.2S和16.6S的RNA种类是核糖体RNA，而高分子量的31.4S、38.0S和48.0S种类是核糖体前体。核糖体RNA合成在从环状体到裂殖体阶段的整个细胞周期中都有发生；[H3]-腺苷的最大掺入发生在核分裂前的滋养体后期。
