Tolley S P, Davies G J, O'Shea M, Cockett M I, Docherty A J, Murphy G
Department of Chemistry, University of York, Heslington, England.
Proteins. 1993 Dec;17(4):435-7. doi: 10.1002/prot.340170410.
A nonglycosylated (N30QN78Q) form of the human tissue inhibitor of metalloproteinases, TIMP-1, has been prepared and crystallized in a form suitable for X-ray diffraction analysis. Small single crystals have been grown using sodium tartrate as a precipitant. The crystals are in space group P2(1), with cell dimensions a = 35.28, b = 53.95, c = 48.56, and beta = 96.0 degrees. There is a single molecule of TIMP-1 in the asymmetric unit. The crystals diffract to at least 2.3 A resolution. Complete data have been collected to 2.9 A and a search for heavy-metal derivatives is in progress.
已制备出人基质金属蛋白酶组织抑制剂TIMP-1的非糖基化（N30QN78Q）形式，并使其结晶为适合X射线衍射分析的形式。使用酒石酸钠作为沉淀剂生长出了小的单晶。这些晶体属于空间群P2(1)，晶胞参数为a = 35.28，b = 53.95，c = 48.56，β = 96.0°。不对称单元中有一个TIMP-1分子。这些晶体的衍射分辨率至少为2.3 Å。已收集到2.9 Å的完整数据，目前正在寻找重金属衍生物。
