截短型组织金属蛋白酶抑制剂δ128 - 194（TIMP - 2）的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of a truncated tissue metalloproteinase inhibitor delta 128-194 TIMP-2.
作者信息
Tolley S, Murphy G, O'Shea M, Ward R, Docherty A, Cockett M, Rawas A, Davies G
机构信息
Department of Chemistry, University of York, Heslington, U.K.
出版信息
J Mol Biol. 1993 Feb 20;229(4):1163-4. doi: 10.1006/jmbi.1993.1114.
Crystals of an active truncated form of a human tissue metallproteinase inhibitor (delta 128-194 TIMP-2), a protein implicated in a number of diseases of the connective tissues, have been grown in a form suitable for study by X-ray diffraction analysis. Crystals grow using polyethylene glycol 600 as a precipitant and are in space group P2(1)2(1)2, with cell dimensions a = 75.28 A, b = 100.77 A, c = 31.45 A. The asymmetric unit appears to contain two molecules of delta TIMP-2 with a Vm value of 2.3 A3/dalton. The crystals diffract to Bragg-spacings beyond 2.5 A. Native data to 3.2 A have been collected and a search for heavy-metal derivatives is in progress.
人组织金属蛋白酶抑制剂（δ128 - 194 TIMP - 2）活性截短形式的晶体已经生长出来，该蛋白质与多种结缔组织疾病有关，其晶体形态适合用于X射线衍射分析研究。晶体生长使用聚乙二醇600作为沉淀剂，属于空间群P2(1)2(1)2，晶胞参数a = 75.28 Å，b = 100.77 Å，c = 31.45 Å。不对称单元似乎包含两个δTIMP - 2分子，Vm值为2.3 Å3/道尔顿。这些晶体的衍射极限可达2.5 Å以上。已经收集了分辨率至3.2 Å的天然数据，并且正在寻找重金属衍生物。
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