矮牵牛叶片外植体茎器官发生过程中产生的苄基腺嘌呤二糖缀合物的鉴定。
Identification of a benzyladenine disaccharide conjugate produced during shoot organogenesis in Petunia leaf explants.
作者信息
Auer C A, Cohen J D
机构信息
Department of Plant Science, University of Connecticut, Storrs 06269.
出版信息
Plant Physiol. 1993 Jun;102(2):541-5. doi: 10.1104/pp.102.2.541.
Prior studies of benzyladenine (BA) metabolism in Petunia hybrida Vilm. leaf explants during shoot organogenesis revealed the presence of an abundant unidentified BA conjugate. The BA conjugate, compound C, made up to 39% of the total pool of BA conjugates in two Petunia lines and was associated with an increased shoot organogenic response when compared with a third Petunia line that did not produce any compound C. Structural analysis of compound C utilizing fast atom bombardment mass spectrometry, two methods of carbohydrate analysis, ultraviolet absorption spectra, and Fourier transform infrared spectra identified it as a new cytokinin conjugate, 6-benzylamino-9-[O-glucopyranosyl-(1-->3)-ribofuranosyl]-purine. Based on our prior biological studies and the similarity of this compound to related cytokinin conjugates, this disaccharide cytokinin conjugate may be part of the interconvertible pool of cytokinins active in Petunia shoot organogenesis.
先前对矮牵牛叶片外植体在芽器官发生过程中苄基腺嘌呤（BA）代谢的研究揭示了一种大量存在的未鉴定BA共轭物。BA共轭物，即化合物C，在两个矮牵牛品系中占BA共轭物总量的39%，与第三个不产生任何化合物C的矮牵牛品系相比，它与芽器官发生反应的增加有关。利用快原子轰击质谱、两种碳水化合物分析方法、紫外吸收光谱和傅里叶变换红外光谱对化合物C进行结构分析，确定它是一种新的细胞分裂素共轭物，即6-苄基氨基-9-[O-吡喃葡萄糖基-(1→3)-呋喃核糖基]-嘌呤。基于我们先前的生物学研究以及该化合物与相关细胞分裂素共轭物的相似性，这种二糖细胞分裂素共轭物可能是矮牵牛芽器官发生中活跃的细胞分裂素可相互转化库的一部分。
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