细胞周期蛋白 - CDK复合物PHO80 - PHO85对转录因子PHO4进行磷酸化。

Phosphorylation of the transcription factor PHO4 by a cyclin-CDK complex, PHO80-PHO85.

作者信息

Kaffman A, Herskowitz I, Tjian R, O'Shea E K

机构信息

School of Medicine, Department of Biochemistry and Biophysics, University of California at San Francisco 94143.

出版信息

Science. 1994 Feb 25;263(5150):1153-6. doi: 10.1126/science.8108735.

DOI:10.1126/science.8108735

PMID:8108735

Abstract

Induction of the yeast gene PHO5 is mediated by the transcription factors PHO2 and PHO4. PHO5 transcription is not detectable in high phosphate; it is thought that the negative regulators PHO80 and PHO85 inactivate PHO2 and PHO4. Here it is reported that PHO80 has homology to yeast cyclins and interacts with PHO85, a p34cdc2/CDC28-related protein kinase. The PHO80-PHO85 complex phosphorylates PHO4; this phosphorylation is correlated with negative regulation of PHO5. These results demonstrate the existence of a cyclin-cdk complex that is used for a regulatory process other than cell-cycle control and identify a physiologically relevant substrate for this complex.

摘要

酵母基因PHO5的诱导由转录因子PHO2和PHO4介导。在高磷酸盐条件下无法检测到PHO5转录；据认为，负调控因子PHO80和PHO85会使PHO2和PHO4失活。本文报道PHO80与酵母细胞周期蛋白具有同源性，并与PHO85相互作用，PHO85是一种与p34cdc2/CDC28相关的蛋白激酶。PHO80 - PHO85复合物使PHO4磷酸化；这种磷酸化与PHO5的负调控相关。这些结果证明存在一种细胞周期蛋白 - 细胞周期蛋白依赖性激酶复合物，其用于细胞周期控制以外的调节过程，并鉴定出该复合物的一个生理相关底物。