细胞周期蛋白依赖性激酶抑制剂PHO81对激酶PHO80-PHO85的磷酸盐调节失活作用

Phosphate-regulated inactivation of the kinase PHO80-PHO85 by the CDK inhibitor PHO81.

作者信息

Schneider K R, Smith R L, O'Shea E K

机构信息

Department of Biochemistry and Biophysics, School of Medicine, University of California, San Francisco 94143.

出版信息

Science. 1994 Oct 7;266(5182):122-6. doi: 10.1126/science.7939631.

DOI:10.1126/science.7939631

PMID:7939631

Abstract

A complex consisting of the cyclin-dependent kinase (CDK) PHO85 and the cyclin PHO80 phosphorylates and is thought to inactivate the transcription factor PHO4 when yeast cells are grown in medium containing high concentrations of phosphate. The CDK inhibitor PHO81 inhibits the kinase activity of the PHO80-PHO85 complex when Saccharomyces cerevisiae cells are grown in medium depleted of phosphate. A region of PHO81 with similarity to the mammalian CDK inhibitor p16INK4 is sufficient for inhibition in vitro. These studies demonstrate that CDK inhibitors are used to regulate kinases involved in processes other than cell cycle control and suggest that the ankyrin repeat motif may be commonly used for interaction with cyclin-CDK complexes.

摘要

当酵母细胞在含有高浓度磷酸盐的培养基中生长时，由细胞周期蛋白依赖性激酶（CDK）PHO85和细胞周期蛋白PHO80组成的复合物会使转录因子PHO4磷酸化，并被认为使其失活。当酿酒酵母细胞在缺乏磷酸盐的培养基中生长时，CDK抑制剂PHO81会抑制PHO80-PHO85复合物的激酶活性。PHO81中与哺乳动物CDK抑制剂p16INK4相似的区域足以在体外发挥抑制作用。这些研究表明，CDK抑制剂可用于调节参与细胞周期控制以外过程的激酶，并提示锚蛋白重复基序可能常用于与细胞周期蛋白-CDK复合物相互作用。