酿酒酵母PHO系统的负调控元件：PHO80与PHO85蛋白之间的相互作用

Negative regulatory elements of the Saccharomyces cerevisiae PHO system: interaction between PHO80 and PHO85 proteins.

作者信息

Gilliquet V, Legrain M, Berben G, Hilger F

机构信息

Unité de Microbiologie, Faculté des Sciences Agronomiques, Gembloux Belgium.

出版信息

Gene. 1990 Dec 15;96(2):181-8. doi: 10.1016/0378-1119(90)90251-l.

DOI:10.1016/0378-1119(90)90251-l

PMID:2269431

Abstract

The negative regulatory genes, PHO80 and PHO85, involved in transcriptional regulation of the yeast repressible acid-phosphatase-encoding gene, PHO5, have been cloned. Expression of PHO80 and PHO85 has been studied by means of lacZ fusions. We show here that these expressions are inorganic phosphate (Pi) independent and that they are controlled by the PHO80 gene product; moreover, PHO80 expression is controlled by PHO85. We also present genetic evidence for an interaction between the PHO80 and PHO85 proteins: increased PHO85 gene dosage partially compensates for the pho80-1 mutation and this effect is allele-specific. The pho80-1 allele has been cloned and sequenced. The mutation changes Gly229 to Asp. This region was shown to be essential for PHO80 function by C-terminal deletion analysis.

摘要

参与酵母可阻遏酸性磷酸酶编码基因PHO5转录调控的负调控基因PHO80和PHO85已被克隆。通过lacZ融合研究了PHO80和PHO85的表达。我们在此表明，这些表达不依赖无机磷酸盐（Pi），且受PHO80基因产物控制；此外，PHO80的表达受PHO85控制。我们还提供了PHO80和PHO85蛋白之间相互作用的遗传学证据：PHO85基因剂量增加可部分补偿pho80-1突变，且这种效应具有等位基因特异性。pho80-1等位基因已被克隆和测序。该突变将Gly229变为Asp。通过C端缺失分析表明该区域对PHO80功能至关重要。