锌对胎儿三毛滴虫影响的电子显微镜研究

Benchimol M, Almeida J C, Lins U, Gonçalves N R, de Souza W

Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.

Antimicrob Agents Chemother. 1993 Dec;37(12):2722-6. doi: 10.1128/AAC.37.12.2722.

At concentrations of 3.1 to 24 mM, zinc inhibits the multiplication of and kills the pathogenic protozoan Tritrichomonas foetus. Transmission electron microscopy showed that the hydrogenosome, a organelle which is involved in the metabolism of pyruvate and the site of formation of molecular hydrogen, constitutes the main site of the initial effect of zinc. The hydrogenosomal vesicle increases its electron density and dimension. Electron spectroscopy imaging and the electron energy loss spectrum showed the presence of zinc, calcium, and oxygen in the electron-dense areas of the hydrogenosome.

在浓度为3.1至24毫摩尔时，锌会抑制致病性原生动物胎儿三毛滴虫的繁殖并将其杀死。透射电子显微镜显示，氢化酶体（一种参与丙酮酸代谢和分子氢形成的细胞器）是锌初始作用的主要部位。氢化酶体囊泡的电子密度和尺寸增加。电子光谱成像和电子能量损失谱显示在氢化酶体的电子致密区域存在锌、钙和氧。