L-苹果酸对精子丙酮酸脱氢酶活性的影响。

Effect of L-malate on pyruvate dehydrogenase activity of spermatozoa.

作者信息

Gerez de Burgos N M, Gallina F, Burgos C, Blanco A

机构信息

Cátedra de Quimica Biológica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.

出版信息

Arch Biochem Biophys. 1994 Feb 1;308(2):520-4. doi: 10.1006/abbi.1994.1073.

DOI:10.1006/abbi.1994.1073

PMID:8109983

Abstract

Presence of L-malate (from 0.5 to 4 mM concentrations) in the medium produces a marked increment of pyruvate consumption by the special type of mitochondria found in the middle piece of spermatozoa (sperm-type mitochondria, STM). Pyruvate uptake by liver mitochondria is not increased by malate. A comparative study on pyruvate dehydrogenase (PDH) of STM and liver mitochondria from mouse, rat, and rabbit showed that 2 mM L-malate does not modify significantly the activity of liver PDH, while it increases markedly that of spermatozoal PDH in the three species. The differential sensitivity to L-malate appears to be a peculiar regulatory property of the PDH complex in the gametes, which contains at least one component (E1, pyruvate decarboxylase, EC 1.2.4.1) known to be a sperm-specific isozyme.

摘要

培养基中存在L-苹果酸（浓度为0.5至4 mM）会使在精子中段发现的特殊类型线粒体（精子型线粒体，STM）对丙酮酸的消耗显著增加。苹果酸不会增加肝线粒体对丙酮酸的摄取。对小鼠、大鼠和兔子的STM和肝线粒体的丙酮酸脱氢酶（PDH）进行的比较研究表明，2 mM L-苹果酸不会显著改变肝PDH的活性，而在这三个物种中，它会显著增加精子PDH的活性。对L-苹果酸的差异敏感性似乎是配子中PDH复合物特有的调节特性，该复合物至少包含一种已知为精子特异性同工酶的成分（E1，丙酮酸脱羧酶，EC 1.2.4.1）。