Ravindranath M H, Ravindranath M H
Acta Histochem. 1975;53(2):203-5.
A simple procedure is deviced to detect chitin in egg shells and integument of parasites using iodinesulfuric acid method. It is observed that the materials do not survive in saturated alkali at 160 degrees C for 15 min, but resist the treatment of 40% KOH at 90 degrees C for 25 min, indicating that the time and temperature required for optimum hydrolysis may differ from material to material. In some preparations, an yellow-green colour was observed, which was occluded and a positive reaction was obtained with acid pretreatment, indicating that incomplete neutralization may prevent the reaction of chitin aggregates with iodine.
设计了一种简单的程序，使用碘硫酸法检测寄生虫卵壳和体表中的几丁质。观察到这些材料在160℃的饱和碱中15分钟不能存活，但能耐受90℃的40%氢氧化钾处理25分钟，这表明最佳水解所需的时间和温度可能因材料而异。在一些制剂中，观察到一种黄绿色，这种颜色被遮盖，经酸预处理后得到阳性反应，这表明不完全中和可能会阻止几丁质聚集体与碘的反应。
