在纤溶酶原、D17Rp17e和震颤蛋白区域进行详细的物理和基因图谱绘制。

Detailed physical and genetic mapping in the region of plasminogen, D17Rp17e, and quaking.

作者信息

Cox R D, Whittington J, Shedlovsky A, Connelly C S, Dove W F, Goldsworthy M, Larin Z, Lehrach H

机构信息

Genome Analysis Laboratory, Imperial Cancer Research Fund, London, UK.

出版信息

Mamm Genome. 1993 Dec;4(12):687-94. doi: 10.1007/BF00357791.

DOI:10.1007/BF00357791

PMID:8111116

Abstract

We present here a detailed physical map encompassing over 600 kb of mouse Chromosome (Chr) 17 in the region of plasminogen, D17Rp17e, and quaking. This region is cloned in yeast artificial chromosomes (YACs). We have identified several CpG islands within this region from pulsed field gel mapping of mouse genomic DNA and YAC DNA. Five new DNA probes have been generated. One, D17Leh514, is a minimum of about 90 kb distal to plasminogen. Four, D17Leh513, D17Leh512, D17Leh511, and D17Leh510, are proximal to D17Rp17e, the closest previously described genetic marker to quaking viable and quaking lethal-1 mutations. We have genetically mapped D17Leh511 to within 0.15 cM of these mutations. The genetic distance to D17Rp17e from D17Leh511 is also 0.15 cM; the physical distance of less than 360 kb (minimum 200 kb) is consistent with an approximation of 2 Mbp per cM.

摘要

我们在此展示了一张详细的物理图谱，该图谱涵盖了小鼠17号染色体（Chr）上纤溶酶原、D17Rp17e和震颤基因所在区域超过600 kb的范围。该区域已克隆到酵母人工染色体（YACs）中。我们通过对小鼠基因组DNA和YAC DNA进行脉冲场凝胶图谱分析，在该区域内鉴定出了几个CpG岛。已产生了五个新的DNA探针。其中一个，D17Leh514，位于纤溶酶原至少约90 kb远的位置。另外四个，D17Leh513、D17Leh512、D17Leh511和D17Leh510，位于D17Rp17e的近端，D17Rp17e是之前描述的与震颤存活和震颤致死-1突变最接近的遗传标记。我们已将D17Leh511基因定位到这些突变的0.15 cM范围内。从D17Leh511到D17Rp17e的遗传距离也是0.15 cM；小于360 kb（最小200 kb）的物理距离与每cM约2 Mbp的近似值相符。