Brown K A, Sutcliffe M J, Steel K P, Brown S D
Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London, UK.
Mamm Genome. 1994 Jan;5(1):11-4. doi: 10.1007/BF00360561.
The mouse olfactory marker protein gene (Omp) maps close to the deafness mutation shaker-1 (sh-1) and has been considered a candidate gene for both sh-1 and its potential human homolog, the deaf-blind syndrome Usher Type I. Using primers devised from the available rat Olfactory Marker Protein gene sequence, we have determined the coding sequence of the mouse gene in both control inbred strains and six shaker-1 mutants. The coding sequence of the mouse Omp gene shows 97% nucleotide identity and 98% amino acid identity with the rat gene sequence. No sequence variants were detected in the coding region of any of the sh-1 mutants, ruling out Omp as the shaker-1 gene.
小鼠嗅觉标记蛋白基因（Omp）定位于与耳聋突变体shaker-1（sh-1）相近的位置，并且被认为是sh-1及其潜在的人类同源基因——致聋致盲综合征I型Usher综合征的候选基因。利用从现有的大鼠嗅觉标记蛋白基因序列设计的引物，我们已经确定了对照近交系和六个shaker-1突变体中小鼠基因的编码序列。小鼠Omp基因的编码序列与大鼠基因序列的核苷酸同一性为97%，氨基酸同一性为98%。在任何一个sh-1突变体的编码区均未检测到序列变异，排除了Omp作为shaker-1基因的可能性。
