Brown K A, Sutcliffe M J, Steel K P, Brown S D
Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, London, United Kingdom.
Genomics. 1992 May;13(1):189-93. doi: 10.1016/0888-7543(92)90219-i.
One thousand sixty-six progeny have been generated from a backcross segregating for the mouse deafness mutation, shaker-1 (sh-1). One thousand fifty-two mice were analyzed for a protein polymorphism segregating for the distal flanking marker, beta-globin (Hbb), and 13 recombinants between Hbb and sh-1 were identified. One thousand eight mice were analyzed for a restriction fragment length polymorphism segregating for the proximal flanking marker, tyrosinase (c), and 54 recombinants between c and sh-1 were identified, completing a panel of 67 recombinant mice from the backcross in the vicinity of the sh-1 mutation. This panel allows the identification of markers closely linked to the sh-1 mutation that may act as start points for a chromosomal walk to the gene. One such marker, the olfactory marker protein gene (Omp), is recombinant with sh-1 in only one mouse from the recombinant panel. Thus, the Omp gene lies 0.1 cM from sh-1, on average, a distance of 200 kb. Haplotype analysis indicates that Omp lies proximal to sh-1.
通过对携带小鼠耳聋突变基因shaker-1（sh-1）的回交群体进行研究，已产生了1066个后代。对1052只小鼠进行了蛋白质多态性分析，该多态性与位于远端侧翼的标记β-珠蛋白（Hbb）相关，共鉴定出13个Hbb与sh-1之间的重组体。对1008只小鼠进行了限制性片段长度多态性分析，该多态性与位于近端侧翼的标记酪氨酸酶（c）相关，共鉴定出54个c与sh-1之间的重组体，从而完成了一个由67只回交重组小鼠组成的群体，这些小鼠位于sh-1突变附近。该群体有助于鉴定与sh-1突变紧密连锁的标记，这些标记可作为向该基因进行染色体步移的起始点。其中一个这样的标记，即嗅觉标记蛋白基因（Omp），在重组群体中仅有一只小鼠与sh-1发生了重组。因此，Omp基因与sh-1平均相距0.1 cM，距离为200 kb。单倍型分析表明Omp位于sh-1的近端。
