Fassi Fihri O, Mohanty J, Elazhary Y
Département de Microbiologie, Immunologie et Maladies contagieuses, Rabat-Instituts, Rabat, Maroc.
Vet Res. 1993;24(6):488-93.
In order to obtain optimal bovine respiratory syncytial virus adsorption to host cells, the effect of several products (fetal bovine serum, bovine serum albumin, ovalbumin and cytochrome c) was studied. The adsorption of the virus to MDBK cells was higher in the presence of 2% than in the presence of 5% fetal bovine serum. Adsorption was inhibited in the presence of bovine serum albumin at concentrations > 0.2% when added before or at the same time as the virus. Ovalbumin and cytochrome c did not inhibit adsorption. These results will allow the study of virus adsorption on cell receptors.
为了使牛呼吸道合胞病毒对宿主细胞的吸附达到最佳效果，研究了几种产品（胎牛血清、牛血清白蛋白、卵清蛋白和细胞色素c）的作用。在2%胎牛血清存在时，病毒对MDBK细胞的吸附高于5%胎牛血清存在时。当在病毒之前或同时加入浓度>0.2%的牛血清白蛋白时，吸附受到抑制。卵清蛋白和细胞色素c不抑制吸附。这些结果将有助于研究病毒在细胞受体上的吸附。
