Peri B A, Theodore C M, Losonsky G A, Fishaut J M, Rothberg R M, Ogra P L
Clin Exp Immunol. 1982 Apr;48(1):91-101.
The contribution of bronchus-associated or gut-associated lymphoid tissues to the development of specific immunologic reactivity in lactating mammary glands was studied by evaluating the effect of the nature of the antigen and the route of immunization on milk antibody content. Groups of pregnant rabbits were immunized with respiratory syncytial virus (RSV) and bovine serum albumin (BSA) administered i.v., per oral (p.o.) or transtracheal (i.t.) routes. The response to RSV was characterized by the regular appearance of IgA anti-RSV in the colostrum, milk, bronchial, and intestinal secretions following p.o. or i.t. immunization, but not after i.v. immunization. RSV-specific IgG appeared in the colostrum, milk, and serum regardless of the route of immunization. On the other hand, the response to BSA by all three routes of immunization was characterized by the appearance of anti-BSA in serum, colostrum and milk which was solely associated with IgG. The anti-BSA isotype did not change during the 30-day nursing period and was not affected by BSA ingestion before or during pregnancy or during nursing. If BSA was reintroduced after 20 days of nursing, a sharp rise in the Ab content of milk occurred in p.o. but not i.v. immunized dams. This increased anti-BSA was also of the IgG isotype. These observations suggest that the isotypes of specific Ab responses in the lactating mammary gland of the rabbit may be determined by the physical and chemical nature of the antigens contacted on respiratory or intestinal mucosal surfaces.
通过评估抗原性质和免疫途径对乳汁抗体含量的影响，研究支气管相关或肠道相关淋巴组织对哺乳期乳腺特异性免疫反应发展的贡献。将怀孕兔分组，通过静脉注射（i.v.）、口服（p.o.）或经气管（i.t.）途径用呼吸道合胞病毒（RSV）和牛血清白蛋白（BSA）进行免疫。对RSV的反应表现为，口服或经气管免疫后，初乳、乳汁、支气管和肠道分泌物中定期出现IgA抗RSV，但静脉注射免疫后则未出现。无论免疫途径如何，RSV特异性IgG都会出现在初乳、乳汁和血清中。另一方面，通过所有三种免疫途径对BSA的反应表现为，血清、初乳和乳汁中出现抗BSA，且仅与IgG相关。抗BSA同种型在30天的哺乳期内没有变化，并且不受怀孕前、怀孕期或哺乳期摄入BSA的影响。如果在哺乳20天后重新引入BSA，口服免疫的母兔乳汁中抗体含量会急剧上升，而静脉注射免疫的母兔则不会。这种增加的抗BSA也是IgG同种型。这些观察结果表明，兔哺乳期乳腺中特异性抗体反应的同种型可能由呼吸道或肠道粘膜表面接触的抗原的物理和化学性质决定。
