Gilliland S E, Speck M L, Morgan C G
Appl Microbiol. 1975 Oct;30(4):541-5. doi: 10.1128/am.30.4.541-545.1975.
Lactobacilli in fecal material from humans, pigs, and chickens were enumerated on lactobacillus selective agar (LBS). In all samples, higher numbers of lac-tobacilli were detected when plates were incubated in a system flushed with CO2 rather than in air. Much higher numbers of bacteria from human feces were detected when the LBS agar plates were incubated anaerobically in a hydrogen-carbon dioxide atmosphere (GasPak) than when incubated in CO2. The bacteria from human feces isolated on LBS agar incubated anaerobically were predominately bifidobacteria. Cultures from all three sources isolated on LBS agar incubated under CO2 were lactobacilli, including Lactobacillus acidophilus. Differences were observed in biochemical characteristics of some of the L. acidophilus isolated from all three sources. Guanine plus cytosine base ratios of deoxyribonucleic acid isolated from L. acidophilus cultures from humans were lower, in most cases, than those from pigs and chickens.
在乳酸菌选择性琼脂（LBS）上对来自人类、猪和鸡粪便中的乳酸菌进行计数。在所有样本中，当平板在充有二氧化碳的系统中培养而非在空气中培养时，检测到的乳酸菌数量更多。当LBS琼脂平板在氢气 - 二氧化碳气氛（气体发生袋）中厌氧培养时，检测到的来自人类粪便的细菌数量比在二氧化碳中培养时多得多。在厌氧培养的LBS琼脂上分离出的来自人类粪便的细菌主要是双歧杆菌。在二氧化碳条件下培养的LBS琼脂上从所有三个来源分离出的培养物都是乳酸菌，包括嗜酸乳杆菌。从所有三个来源分离出的一些嗜酸乳杆菌的生化特性存在差异。从人类嗜酸乳杆菌培养物中分离出的脱氧核糖核酸的鸟嘌呤加胞嘧啶碱基比率在大多数情况下低于从猪和鸡中分离出的。
