Macadam A J, Ferguson G, Fleming T, Stone D M, Almond J W, Minor P D
National Institute for Biological Standards and Control, Herts, UK.
EMBO J. 1994 Feb 15;13(4):924-7. doi: 10.1002/j.1460-2075.1994.tb06336.x.
Viral protein synthesis in poliovirus infected cells was found to be influenced by mutations in part of the viral 5'-non-coding region (NCR) in a temperature dependent manner. At elevated temperatures these mutations resulted in virus titre reductions that allowed selection of revertant viruses. Some revertants were found to have retained the 5'-NCR mutations but had compensating mutations in the 2A protease gene that were responsible for the suppression of the temperature sensitive phenotypes. The mutations in 2A enhanced viral protein synthesis at a stage when cap dependent translation was already abolished, suggesting that the virally encoded protein 2A is directly involved in the process of cap independent translation in addition to its role in abolishing cap dependent translation.
研究发现，脊髓灰质炎病毒感染细胞中的病毒蛋白合成受到病毒5'-非编码区（NCR）部分区域突变的温度依赖性影响。在较高温度下，这些突变导致病毒滴度降低，从而能够筛选出回复病毒。一些回复病毒被发现保留了5'-NCR突变，但在2A蛋白酶基因中具有补偿性突变，这些突变负责抑制温度敏感表型。2A中的突变在帽依赖性翻译已经被废除的阶段增强了病毒蛋白合成，这表明病毒编码的蛋白2A除了在废除帽依赖性翻译中发挥作用外，还直接参与了不依赖帽的翻译过程。
