Wehmeier U F
Bergische Universität GH Wuppertal, Germany.
Gene. 1995 Nov 7;165(1):149-50. doi: 10.1016/0378-1119(95)00513-6.
Four new shuttle vectors for Escherichia coli (Ec) and Streptomyces, pUWL218, pUWL219, pUWL-SK and pUWL-KS, which permit recognition of recombinant (re-) plasmids on XGal plates in Ec, were constructed. These vectors contain the replication functions of the Streptomyces wide-host-range multicopy plasmid pIJ101, the tsr gene conferring resistance to thiostrepton in Streptomyces, the ColEI origin of replication from the pUC plasmids for replication in Ec and the bla gene conferring resistance to ampicillin in Ec. They possess multiple cloning sites with a number of unique restriction sites and allow direct sequencing of re-derivatives using the pUC sequencing primers.
构建了四种用于大肠杆菌（Ec）和链霉菌的新型穿梭载体pUWL218、pUWL219、pUWL-SK和pUWL-KS，它们能够在XGal平板上识别Ec中的重组（re-）质粒。这些载体包含链霉菌广宿主范围多拷贝质粒pIJ101的复制功能、赋予链霉菌对硫链丝菌素抗性的tsr基因、来自pUC质粒的用于在Ec中复制的ColEI复制起点以及赋予Ec对氨苄青霉素抗性的bla基因。它们具有多个带有许多独特限制酶切位点的多克隆位点，并允许使用pUC测序引物对重新衍生的质粒进行直接测序。
