Stevenson D, Colman K L, Davison A J
MRC Virology Unit, University of Glasgow, U.K.
J Gen Virol. 1994 Feb;75 ( Pt 2):317-26. doi: 10.1099/0022-1317-75-2-317.
The proteins predicted to be encoded by varicella-zoster virus (VZV) genes 47 and 66 display sequence similarity to the serine/threonine family of protein kinases. Homologues of gene 47 exist in alpha-, beta- and gamma-herpesviruses but homologues of gene 66 are specific to the alpha-herpesviruses. Monospecific rabbit antisera were raised against two separate fusion proteins constructed from a portion of each protein fused to the carboxy terminus of beta-galactosidase. These antisera were used to characterize the 47 and 66 proteins in VZV-infected cells and in cells infected with vaccinia virus recombinants expressing each protein. The 47 proteins is a 54K phosphoprotein which is distributed between the cytoplasmic and nuclear compartments of VZV-infected cells and is associated with the capsid/tegument fraction of purified VZV particles. Gene 66 encodes a 48K phosphoprotein when expressed by VZV or a vaccinia virus recombinant, and, in the latter case, the 66 protein was located exclusively in the cytoplasm. The 47 protein immunoprecipitated from VZV-infected cells could be phosphorylated in vitro, but the same protein produced by in vitro transcription and translation could not. This and other evidence indicates that additional proteins induced or encoded by VZV may be involved in the phosphorylation of the 47 protein.
水痘带状疱疹病毒（VZV）基因47和66预测编码的蛋白质与丝氨酸/苏氨酸蛋白激酶家族具有序列相似性。基因47的同源物存在于α、β和γ疱疹病毒中，但基因66的同源物是α疱疹病毒特有的。针对由与β-半乳糖苷酶羧基末端融合的每种蛋白质的一部分构建的两种单独的融合蛋白，制备了单特异性兔抗血清。这些抗血清用于鉴定VZV感染细胞以及感染表达每种蛋白质的痘苗病毒重组体的细胞中的47和66蛋白。47蛋白是一种54K的磷蛋白，分布于VZV感染细胞的细胞质和细胞核区室，并与纯化的VZV颗粒的衣壳/被膜部分相关。当由VZV或痘苗病毒重组体表达时，基因66编码一种48K的磷蛋白，在后一种情况下，66蛋白仅位于细胞质中。从VZV感染细胞中免疫沉淀的47蛋白可在体外被磷酸化，但通过体外转录和翻译产生的相同蛋白则不能。这一证据以及其他证据表明，VZV诱导或编码的其他蛋白质可能参与47蛋白的磷酸化。
