Ramamurthy T, Albert M J, Huq A, Colwell R R, Takeda Y, Takeda T, Shimada T, Mandal B K, Nair G B
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
J Med Microbiol. 1994 Mar;40(3):194-6. doi: 10.1099/00222615-40-3-194.
A collection of 13 strains of Vibrio mimicus, including both clinical and environmental isolates from different geographic regions, was examined for various toxins. One strain of environmental origin produced cholera-like toxin (CT) which was completely absorbed with anti-CT immunoglobulin G, five strains produced a haemolysin that cross-reacted with the thermostable direct haemolysin of V. parahaemolyticus and DNA from two strains hybridised with a DNA probe specific for the heat-stable enterotoxin of V. cholerae non-O1. Culture supernates of all strains produced a factor that was cytotoxic to Vero and Chinese hamster ovary cells. In this study, we were able to identify strains of V. mimicus that produced, or had the genetic potential to produce, several toxin types simultaneously. The role of these strains as genetic reservoirs is discussed.
对13株拟态弧菌进行了检测，这些菌株包括来自不同地理区域的临床分离株和环境分离株，检测其是否产生各种毒素。一株环境来源的菌株产生了霍乱样毒素（CT），该毒素能被抗CT免疫球蛋白G完全吸收；五株菌株产生了一种溶血素，该溶血素与副溶血性弧菌的耐热直接溶血素有交叉反应；两株菌株的DNA与霍乱弧菌非O1型耐热肠毒素的特异性DNA探针杂交。所有菌株的培养上清液都产生了一种对Vero细胞和中国仓鼠卵巢细胞具有细胞毒性的因子。在本研究中，我们能够鉴定出同时产生或具有产生多种毒素类型遗传潜力的拟态弧菌菌株。讨论了这些菌株作为基因库的作用。
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