冈田酸对大鼠肥大细胞免疫性和非免疫性组胺释放的影响。
Effect of okadaic acid on immunologic and non-immunologic histamine release in rat mast cells.
作者信息
Estévez M D, Vieytes M R, Louzao M C, Botana L M
机构信息
Departamento de Farmacología, Facultad de Veterinaria, Lugo, Spain.
出版信息
Biochem Pharmacol. 1994 Feb 9;47(3):591-3. doi: 10.1016/0006-2952(94)90194-5.
We have studied the effect of the protein phosphatase (PP) inhibitor, okadaic acid (OKA) on histamine release elicited by immunologic and non-immunologic stimuli in peritoneal and pleural rat mast cells. When cells were stimulated with antigen (egg albumin), OKA strongly inhibited histamine release. This finding suggests that PP1 and PP2A substrates mediate immunoglobulin E-(IgE) dependent secretion in mast cells. In contrast, after non-immunologic activation of mast cells with different drugs, such as the neuropeptide growth hormone releasing factor (GRF) and the cytostatic agents Adriamycin, navelbine and mitoxantrone, there is no effect of OKA on histamine secretion. These results indicate that IgE-dependent secretion is mediated by substrates of PP1 and PP2A, whereas following non-immunological stimuli, they activate pathways that lead to protein phosphorylation; these proteins are not substrates of PP1 and PP2A.
我们研究了蛋白磷酸酶（PP）抑制剂冈田酸（OKA）对大鼠腹膜和胸膜肥大细胞中免疫和非免疫刺激引发的组胺释放的影响。当用抗原（卵清蛋白）刺激细胞时，OKA强烈抑制组胺释放。这一发现表明，PP1和PP2A的底物介导肥大细胞中免疫球蛋白E-（IgE）依赖性分泌。相反，在用不同药物（如神经肽生长激素释放因子（GRF）和细胞抑制剂阿霉素、长春瑞滨和米托蒽醌）对肥大细胞进行非免疫激活后，OKA对组胺分泌没有影响。这些结果表明，IgE依赖性分泌由PP1和PP2A的底物介导，而在非免疫刺激后，它们激活导致蛋白质磷酸化的途径；这些蛋白质不是PP1和PP2A的底物。
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