Collin T, Lory P, Taviaux S, Courtieu C, Guilbault P, Berta P, Nargeot J
Centre de Recherches de Biochimie Macromoléculaire, CNRS UPR9008, INSERM U249, Montpellier, France.
Eur J Biochem. 1994 Feb 15;220(1):257-62. doi: 10.1111/j.1432-1033.1994.tb18621.x.
A novel human-voltage-dependent-calcium-channel (VDCC) beta subunit was isolated from a 9-week-old human total-embryo cDNA library. Of the four genes encoding beta-subunit isoforms that have been identified in animal species, this isoform shares strong similarity with the rat and rabbit beta 3-related gene product and is referred to here as H beta 3 subunit. The H beta 3 isoform is the second beta subunit identified in human. Its open reading frame encodes a 482-amino-acid protein with a predicted molecular mass of 54.571 kDa. The H beta 3 mRNA is expressed mostly in brain, smooth muscle and ovary. The gene for the human H beta 3 was specifically localized on chromosome 12q13. The cloned H beta 3 subunit was further expressed in Xenopus oocytes to demonstrate its ability to modulate VDCC activity.
从一个9周龄的人类全胚胎cDNA文库中分离出一种新型的人类电压依赖性钙通道（VDCC）β亚基。在动物物种中已鉴定出的编码β亚基同工型的四个基因中，这种同工型与大鼠和兔子的β3相关基因产物具有很强的相似性，在这里被称为Hβ3亚基。Hβ3同工型是在人类中鉴定出的第二个β亚基。其开放阅读框编码一个482个氨基酸的蛋白质，预测分子量为54.571 kDa。Hβ3 mRNA主要在脑、平滑肌和卵巢中表达。人类Hβ3基因被特异性定位于12号染色体q13区。克隆的Hβ3亚基在非洲爪蟾卵母细胞中进一步表达，以证明其调节VDCC活性的能力。
