Balczon R, Bao L, Zimmer W E
Department of Structural and Cellular Biology, University of South Alabama, Mobile 36688.
J Cell Biol. 1994 Mar;124(5):783-93. doi: 10.1083/jcb.124.5.783.
We report the identification and primary sequence of PCM-1, a 228-kD centrosomal protein that exhibits a distinct cell cycle-dependent association with the centrosome complex. Immunofluorescence microscopy using antibodies against recombinant PCM-1 demonstrated that PCM-1 is tightly associated with the centrosome complex through G1, S, and a portion of G2. However, late in G2, as cells prepare for mitosis, PCM-1 dissociates from the centrosome and then remains dispersed throughout the cell during mitosis before re-associating with the centrosomes in the G1 phase progeny cells. These results demonstrate that the pericentriolar material is a dynamic substance whose composition can fluctuate during the cell cycle.
我们报告了PCM-1的鉴定结果及其一级序列，PCM-1是一种228-kD的中心体蛋白，与中心体复合体呈现出明显的细胞周期依赖性关联。使用抗重组PCM-1抗体进行的免疫荧光显微镜检查表明，PCM-1在G1期、S期以及部分G2期与中心体复合体紧密结合。然而，在G2期后期，当细胞为有丝分裂做准备时，PCM-1从中心体解离，然后在有丝分裂期间一直分散于整个细胞中，直至在G1期子代细胞中重新与中心体结合。这些结果表明，中心粒周围物质是一种动态物质，其组成在细胞周期中会发生波动。
