Mizutamari R K, Wiegandt H, Nores G A
Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
J Neuroimmunol. 1994 Mar;50(2):215-20. doi: 10.1016/0165-5728(94)90048-5.
Samples of plasma from normal human adults were screened for anti-ganglioside antibodies by HPTLC-immunostaining and enzyme-linked immunosorbent assay (ELISA). All the samples analyzed showed IgM-immunoreactivity to GM1 ganglioside and its related glycolipid GA1. Reactivity to GD1b, GM2 and LM1 was also detected in 85, 80 and 20% of the analyzed samples, respectively. The main immunoreactivity is related to the asialo-ganglioside GA1. Using inhibition by soluble antigen and affinity chromatography techniques it was possible to distinguish two populations of anti-GA1 antibodies. One with high affinity reacting only with GA1, and another with low affinity reacting also with GM1 and GD1b. The antibodies that recognize GM2 are of low affinity and appear to be different from those that react with GA1. We postulate that the anti-GM1/GD1b immunoreactivity present in normal human plasma could be a cross-reaction of antibodies originally directed to a GA1-like carbohydrate structure. Anti-GA1 and anti-GM1 titers were calculated as the reciprocal of the plasma dilution needed to obtain the half maximal antibody binding, a titer definition that we consider more suitable to compare data from different laboratories than those usually employed.
采用高效薄层层析免疫染色法和酶联免疫吸附测定（ELISA）对正常成年人类的血浆样本进行抗神经节苷脂抗体筛查。所有分析样本均显示出对GM1神经节苷脂及其相关糖脂GA1的IgM免疫反应性。在分别85%、80%和20%的分析样本中也检测到了对GD1b、GM2和LM1的反应性。主要免疫反应性与去唾液酸神经节苷脂GA1有关。利用可溶性抗原抑制和亲和层析技术，能够区分出两类抗GA1抗体。一类具有高亲和力，仅与GA1反应；另一类具有低亲和力，也与GM1和GD1b反应。识别GM2的抗体亲和力较低，似乎与那些与GA1反应的抗体不同。我们推测，正常人类血浆中存在的抗GM1/GD1b免疫反应性可能是原本针对类似GA1碳水化合物结构的抗体的交叉反应。抗GA1和抗GM1滴度计算为获得最大抗体结合量一半时所需血浆稀释倍数的倒数，我们认为这种滴度定义比通常使用的定义更适合比较不同实验室的数据。
