Hitoshi S, Kusunoki S, Kon K, Chiba A, Waki H, Ando S, Kanazawa I
Department of Neurology, Faculty of Medicine, University of Tokyo, Japan.
J Neuroimmunol. 1996 May;66(1-2):95-101. doi: 10.1016/0165-5728(96)00024-0.
A hitherto undescribed ganglioside was detected in a crude ganglioside fraction of bovine brain using an IgM M-protein binding to Gal beta 1, 3GalNAc residue. We purified and identified it as 9-O-acetyl GD1b based on results of alkali treatment that yielded GD1b and results of fast atom bombardment-mass and gas chromatography-mass spectrometries. 9-O-acetyl GD1b was also found to be present in human peripheral nerve tissue. The reactivities of the serum antibodies from patients with Guillain-Barré syndrome to 9-O-acetyl GD1b, GD1b, and GM1 were determined by ELISA and TLC immunostaining. Nineteen of 85 serum samples from Guillain-Barré syndrome patients had antibodies that bound to 9-O-acetyl GD1b: 14 of the positive samples also reacted with GM1 and GD1b, three reacted with GM1 but not with GD1b, one with GD1b but not with GM1, and one with neither GM1 nor GD1b. These results show that a subset of patients with Guillain-Barré syndrome had antibodies that react with 9-O-acetyl GD1b; therefore, this ganglioside can serve as a target antigen against the antibodies present in Guillain-Barré syndrome.
利用一种与Galβ1,3GalNAc残基结合的IgM M蛋白，在牛脑粗制神经节苷脂组分中检测到一种迄今未被描述的神经节苷脂。基于碱处理产生GD1b的结果以及快原子轰击质谱和气相色谱 - 质谱分析结果，我们对其进行了纯化并鉴定为9 - O - 乙酰基GD1b。还发现9 - O - 乙酰基GD1b存在于人类外周神经组织中。通过酶联免疫吸附测定（ELISA）和薄层层析免疫染色法测定了吉兰 - 巴雷综合征患者血清抗体对9 - O - 乙酰基GD1b、GD1b和GM1的反应性。85份吉兰 - 巴雷综合征患者血清样本中有19份含有与9 - O - 乙酰基GD1b结合的抗体：其中14份阳性样本也与GM1和GD1b反应，3份与GM1反应但不与GD1b反应，1份与GD1b反应但不与GM1反应，1份既不与GM1也不与GD1b反应。这些结果表明，一部分吉兰 - 巴雷综合征患者具有与9 - O - 乙酰基GD1b反应的抗体；因此，这种神经节苷脂可作为针对吉兰 - 巴雷综合征中存在的抗体的靶抗原。
