Sereno M M, Gorziglia M I
Epidemiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Virology. 1994 Mar;199(2):500-4. doi: 10.1006/viro.1994.1153.
The nucleotide and deduced amino acid sequence of the gene 4 of murine rotavirus strain Eb were determined. The gene is 2359 nucleotides in length and encodes for a protein of 775 amino acids. Comparison of the VP4 amino acid sequence of the Eb strain with several human and animal rotavirus strains which represent all of the currently recognized distinct VP4 genotypes revealed amino acid identities of from 55.7-75.1% for VP4, 37.1-63.3% for VP8, and 23.9-52.1% for the B region (amino acids 84-180). In addition, antisera to recombinant VP4s of five distinct rotavirus serotypes and two subtypes failed to react significantly by neutralization assay with the Eb strain. Thus, it appears that the Eb strain should be considered a new VP4 genotype and/or serotype.
测定了小鼠轮状病毒Eb株基因4的核苷酸序列和推导的氨基酸序列。该基因长度为2359个核苷酸，编码一个由775个氨基酸组成的蛋白质。将Eb株的VP4氨基酸序列与代表目前所有已识别的不同VP4基因型的几个人类和动物轮状病毒株进行比较，结果显示VP4的氨基酸同一性为55.7 - 75.1%，VP8为37.1 - 63.3%，B区域（氨基酸84 - 180）为23.9 - 52.1%。此外，针对五种不同轮状病毒血清型和两种亚型的重组VP4的抗血清，通过中和试验与Eb株没有明显反应。因此，Eb株似乎应被视为一种新的VP4基因型和/或血清型。
