Griffin D D, Kirkwood C D, Parashar U D, Woods P A, Bresee J S, Glass R I, Gentsch J R
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 2000 Jul;38(7):2784-7. doi: 10.1128/JCM.38.7.2784-2787.2000.
Rotavirus strains from 964 fecal specimens collected from children at 11 U.S. hospital laboratories from November 1997 to March 1998 and from samples collected at 12 laboratories from November 1998 to March 1999 were typed for G and P proteins. Serotype G1 was the predominant serotype in 1997-1998 (88%), followed by G2 (6.2%), G9 (3.3%), and G3 (1.5%). This pattern was similar to that seen in 1998-1999: G1 (79%), G2 (15%), G9 (3.0%), G4 (1.6%), and G3 (0.3%). Novel P[9] strains were identified in both seasons, and analysis of a 364-nucleotide fragment from gene segment 4 of one of the strains demonstrated 97.3% nucleotide identity with the prototype P3[9],G3 strain, AU1, isolated in Japan. This is the first report of a human AU1-like strain in the United States. These results reinforce our initial findings that serotype G9 persists in the United States but has not become a predominant strain and that the common serotypes G1 to G4 account for almost 90% of strains in circulation. Other uncommon strains exist in the United States but may have been overlooked before because of their low prevalence and the use of inadequate diagnostic tools.
对1997年11月至1998年3月期间从美国11家医院实验室的儿童中收集的964份粪便标本以及1998年11月至1999年3月期间从12家实验室收集的样本中的轮状病毒株进行了G蛋白和P蛋白分型。血清型G1是1997 - 1998年的主要血清型（88%），其次是G2（6.2%）、G9（3.3%）和G3（1.5%）。这种模式与1998 - 1999年观察到的相似：G1（79%）、G2（15%）、G9（3.0%）、G4（1.6%）和G3（0.3%）。在两个季节中均鉴定出新型P[9]株，对其中一株的基因片段4的364个核苷酸片段进行分析，结果显示与在日本分离的原型P3[9]，G3株AU1具有97.3%的核苷酸同一性。这是美国首次报告人类AU1样株。这些结果强化了我们最初的发现，即血清型G9在美国持续存在，但尚未成为主要毒株，常见血清型G1至G4占流行毒株的近90%。美国存在其他不常见的毒株，但由于其低流行率和诊断工具不足，以前可能被忽视了。
