Midy V, Plouët J
Laboratoire de Biologie Moléculaire Eucaryote, UPR 9006 CNRS, Toulouse, France.
Biochem Biophys Res Commun. 1994 Feb 28;199(1):380-6. doi: 10.1006/bbrc.1994.1240.
Vasculotropin/vascular endothelial growth factor (VAS/VEGF) is an angiogenic growth factor whose biological activity seems to be restricted in vitro to vascular endothelial cells. We describe here that fetal bovine osteoblasts (OB) bind VAS/VEGF but do not proliferate upon its addition. However VAS/VEGF induces migration, PTH-dependent cAMP accumulation and alkaline phosphatase increase when added to OB. The maximal effects reach levels comparable to that obtained with bone morphogenetic protein 2 (BMP-2), although the VAS/VEGF concentrations required are at least 100 fold lower. Our results suggest that VAS/VEGF could be an important regulator of osteoblastic differentiation.
血管生长素/血管内皮生长因子（VAS/VEGF）是一种血管生成生长因子，其生物学活性在体外似乎仅限于血管内皮细胞。我们在此描述，胎牛成骨细胞（OB）能结合VAS/VEGF，但添加该因子后细胞并不增殖。然而，将VAS/VEGF添加到OB中时，它会诱导细胞迁移、甲状旁腺激素（PTH）依赖性环磷酸腺苷（cAMP）积累以及碱性磷酸酶增加。尽管所需的VAS/VEGF浓度至少低100倍，但最大效应达到了与骨形态发生蛋白2（BMP-2）相当的水平。我们的结果表明，VAS/VEGF可能是成骨细胞分化的重要调节因子。
