Zampatti O, Roques C, Michel G
Laboratory of Bacteriology, Virology, and Industrial Microbiology, Faculty of Pharmacy, Paul Sabatier University, Toulouse, France.
Caries Res. 1994;28(1):35-42. doi: 10.1159/000261618.
The development of an in vitro model simulating the oral cavity has allowed the antiplaque activity of a toothpaste containing 0.004% chlorhexidine to be tested. The model is based on the continuous irrigation of bovine tooth samples with artificial saliva. Scanning electron microscopy showed homogeneous colonization of monobacterial plaque by Streptococcus mutans on dental surfaces under these model conditions. The enamel was colonized with regular cell clusters showing a globular appearance. The globules coalesced with increasing plaque thickness. Treatment with toothpaste containing 0.004% chlorhexidine showed a more marked inhibition of bacterial colonization on enamel surfaces than placebo paste lacking this antimicrobial agent. These results indicate that the toothpaste formulation is compatible with chlorhexidine. It is concluded that the in vitro model developed in this study is suitable for the testing of the antiplaque activity of antimicrobial agents, particularly when in a semisolid form such as in a toothpaste.
一种模拟口腔的体外模型的开发，使得对含有0.004%氯己定的牙膏的防龋斑活性进行测试成为可能。该模型基于用人造唾液持续冲洗牛牙样本。扫描电子显微镜显示，在这些模型条件下，变形链球菌在牙齿表面形成了均匀的单菌种菌斑定植。牙釉质上有规则的细胞簇定植，呈球状外观。随着菌斑厚度增加，这些小球会融合在一起。与不含这种抗菌剂的安慰剂牙膏相比，含有0.004%氯己定的牙膏对牙釉质表面细菌定植的抑制作用更为显著。这些结果表明该牙膏配方与氯己定相容。得出的结论是，本研究中开发的体外模型适用于测试抗菌剂的防龋斑活性，特别是当抗菌剂呈半固体形式（如牙膏）时。
