Haycox C L, Ratner B D
Center for Bioengineering, University of Washington, Seattle 98195.
J Biomed Mater Res. 1993 Sep;27(9):1181-93. doi: 10.1002/jbm.820270909.
A short-term in vitro test to study platelet interactions with biomaterials is described. Using fresh human blood and a modified Chandler loop system, beta-thromboglobulin release was measured. Also, adherent platelets were observed by using scanning electron microscopy (SEM) and a colorimetric stain specific for human platelet GPIIIa. Materials studied in these experiments were polyethylene (PE), Biomer, poly(vinyl alcohol) (PVA), and a polyurethane prepared with octadecyl pendant groups (ODCE). Four blood reactions were observed: (1) Platelets continually adhere and activate on the Biomer; (2) platelets initially adhere and activate but then spread to a thin, passivating film on the PE; (3) platelets do not adhere to the PVA surface but continually react with it upon contact; and (4) platelets neither adhere to nor activate on the ODCE surface. Reactions (2) and (4) are considered characteristic of blood-compatible materials.
描述了一种用于研究血小板与生物材料相互作用的短期体外试验。使用新鲜人血和改良的钱德勒环路系统，测量了β-血小板球蛋白的释放。此外，通过扫描电子显微镜（SEM）和针对人血小板糖蛋白IIIa的比色染色观察粘附的血小板。这些实验中研究的材料有聚乙烯（PE）、Biomer、聚乙烯醇（PVA）以及一种带有十八烷基侧基的聚氨酯（ODCE）。观察到四种血液反应：（1）血小板在Biomer上持续粘附并激活；（2）血小板最初粘附并激活，但随后在PE上扩展形成一层薄的钝化膜；（3）血小板不粘附于PVA表面，但接触后会持续与之反应；（4）血小板既不粘附于ODCE表面也不在其上激活。反应（2）和（4）被认为是血液相容性材料的特征。
