Ryan I P, Schriock E D, Taylor R N
Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco 94143.
J Clin Endocrinol Metab. 1994 Mar;78(3):642-9. doi: 10.1210/jcem.78.3.8126136.
Endometriosis is a common gynecological disorder of unclear pathogenesis. We have established an in vitro model to investigate phenotypic similarities and differences between normal endometrial and endometriosis cells. Highly purified cultures of epithelial and stromal cells were isolated from normal endometrium and endometriosis implants. Morphological features as well as immunocytochemical markers confirm these isolates as epithelial and stromal cells. Potential hormone responsiveness was established by the documentation of estrogen receptor mRNA in epithelial and stromal cells isolated from both tissue types. Expression of this receptor protein was verified in stromal cells by competitive radioligand binding, revealing comparable receptor numbers and dissociation constants. CA-125 is selectively secreted in similar concentrations by epithelial cells isolated from both tissue types. PRL secretion is selectively exhibited by progestin-stimulated stromal cells from both tissue types. Our findings demonstrate that highly purified epithelial and stromal cells cultured from normal endometrial and endometriosis tissues express the same phenotypic and functional markers as their in vivo counterparts. These cultures provide useful models to identify endometriosis-specific cell products that contribute to the pathogenesis of this disorder.
子宫内膜异位症是一种发病机制不明的常见妇科疾病。我们建立了一个体外模型，以研究正常子宫内膜细胞和子宫内膜异位症细胞之间的表型异同。从正常子宫内膜和子宫内膜异位症植入物中分离出高度纯化的上皮细胞和基质细胞培养物。形态学特征以及免疫细胞化学标记物证实这些分离物为上皮细胞和基质细胞。通过记录从两种组织类型中分离出的上皮细胞和基质细胞中的雌激素受体mRNA，确定了潜在的激素反应性。通过竞争性放射性配体结合在基质细胞中验证了该受体蛋白的表达，显示出相当的受体数量和解离常数。两种组织类型分离出的上皮细胞以相似的浓度选择性分泌CA-125。两种组织类型的孕激素刺激的基质细胞均选择性地表现出PRL分泌。我们的研究结果表明，从正常子宫内膜和子宫内膜异位症组织培养的高度纯化的上皮细胞和基质细胞表达与其体内对应物相同的表型和功能标记物。这些培养物为鉴定导致该疾病发病机制的子宫内膜异位症特异性细胞产物提供了有用的模型。
