Banerji S, Wakefield A E, Allen A G, Maskell D J, Peters S E, Hopkin J M
University of Oxford Department of Paediatrics, John Radcliffe Hospital, Headington, UK.
J Gen Microbiol. 1993 Dec;139(12):2901-14. doi: 10.1099/00221287-139-12-2901.
The arom gene, encoding a single polypeptide that catalyses five consecutive steps of the pre-chorismate aromatic amino acid biosynthetic pathway, has been cloned from the opportunistic pathogen Pneumocystis carinii. There is a single open reading frame of 4788 bp which includes an intron of 45 bp that does not introduce a stop codon into the sequence. Thus, the derived amino acid sequence consists of 1581 residues, which is highly homologous to all fungal AROM proteins studied to date. These data support the view that P. carinii is a fungus and imply that its aromatic amino acid biosynthesis is conventionally organized.
芳香基因编码一种单一多肽，该多肽催化分支酸前体芳香族氨基酸生物合成途径的连续五个步骤，已从机会致病菌卡氏肺孢子虫中克隆得到。有一个4788 bp的单一开放阅读框，其中包括一个45 bp的内含子，该内含子未在序列中引入终止密码子。因此，推导的氨基酸序列由1581个残基组成，与迄今为止研究的所有真菌AROM蛋白高度同源。这些数据支持卡氏肺孢子虫是一种真菌的观点，并表明其芳香族氨基酸生物合成是按照常规方式组织的。
