Bode G, Mauch F, Ditschuneit H, Malfertheiner P
Department of Internal Medicine I, University of Ulm, Germany.
J Gen Microbiol. 1993 Dec;139(12):3029-33. doi: 10.1099/00221287-139-12-3029.
For the first time polyphosphate (poly P) granules have been detected in Helicobacter pylori organisms colonizing the gastric antrum as well as in organisms isolated from the same tissue. Poly P granules showed typical sublimation characteristics during exposure to the electron beam and chipped out of ultrathin sectioning. A prominent phosphorus signal was identified using elemental specific electron microscopy such as electron energy loss spectroscopy (EELS) and was localized to at least three different locations: the cytoplasm, the flagellar pole and in association with the cell membrane. Intracytoplasmatic structures had a diameter of 0.05-0.2 micron, whereas the structures near the flagellar pole were much smaller (0.02 micron). The membrane-associated phosphate aggregates were visible only after staining with Pb(NO3)2 or with electron spectroscopic imaging (ESI). Poly P granules seem to be important energy and phosphorus stores and it is thought that they participate in the regulation of various and distinct metabolic processes of H. pylori.
首次在定殖于胃窦的幽门螺杆菌生物体以及从同一组织分离出的生物体中检测到多聚磷酸盐（poly P）颗粒。多聚磷酸盐颗粒在电子束照射下呈现出典型的升华特征，并从超薄切片中脱落。使用诸如电子能量损失谱（EELS）等元素特异性电子显微镜鉴定出一个显著的磷信号，该信号定位于至少三个不同位置：细胞质、鞭毛极以及与细胞膜相关联的部位。细胞质内结构的直径为0.05 - 0.2微米，而鞭毛极附近的结构要小得多（0.02微米）。仅在用Pb(NO3)2染色或电子光谱成像（ESI）后才能看到与膜相关的磷酸盐聚集体。多聚磷酸盐颗粒似乎是重要的能量和磷储存库，并且据认为它们参与幽门螺杆菌各种不同代谢过程的调节。
