Kool M, Broer R, Zuidema D, Goldbach R W, Vlak J M
Department of Virology, Wageningen Agricultural University, The Netherlands.
J Gen Virol. 1994 Mar;75 ( Pt 3):487-94. doi: 10.1099/0022-1317-75-3-487.
The nucleotide sequence and genetic organization of a 7297 bp region within the EcoRI-C fragment of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV) are presented. Eight putative open reading frames were found and their respective amino acid sequences compared with a number of data libraries. ORF 1227 corresponded with gp41 and its predicted protein sequence was found to be 55 amino acids longer at its C terminus than reported previously. Moreover the main part of the ORF 1227 product, including the additional 55 amino acids, showed a high degree of homology with protein p40 of Helicoverpa zea single nucleocapsid nuclear polyhedrosis virus (HzSNPV). Three other ORFs in the analysed AcMNPV region showed homology with ORFs in the HzSNPV sequence, indicating that the general organization of this region is similar in both viruses. However one ORF found in the AcMNPV sequence was absent from the corresponding HzSNPV sequence.
本文介绍了苜蓿银纹夜蛾多粒包埋核型多角体病毒（AcMNPV）EcoRI-C片段内一个7297 bp区域的核苷酸序列和基因组织。发现了8个推定的开放阅读框，并将它们各自的氨基酸序列与多个数据库进行了比较。ORF 1227与gp41相对应，其预测的蛋白质序列在C末端比先前报道的长55个氨基酸。此外，ORF 1227产物的主要部分，包括额外的55个氨基酸，与玉米螟单粒包埋核型多角体病毒（HzSNPV）的p40蛋白具有高度同源性。分析的AcMNPV区域中的其他三个ORF与HzSNPV序列中的ORF具有同源性，表明这两个病毒中该区域的总体组织相似。然而，在AcMNPV序列中发现的一个ORF在相应的HzSNPV序列中不存在。
