Gopalakrishnan P V
Immunol Commun. 1975;4(5):499-506. doi: 10.3109/08820137509057337.
Affinity labeling of rabbit IgG anti-lactose antibodies of restricted heterogeneity by bromacetylaminolac dye was carried out in dark and the label on the isolated chains determined by SDS gel electrophoresis. The pattern of labeling was compared with that obtained when the reaction was carried out in light using the same reagent. Differences were observed in the mode of labeling of chains. Possible use of mapping the active site of the antibody by the same labeling reagent which can exist in two geometrical forms under dark and light conditions is discussed.
在黑暗中用溴乙酰氨基乳糖染料对具有有限异质性的兔抗乳糖IgG抗体进行亲和标记，并通过SDS凝胶电泳确定分离链上的标记。将标记模式与使用相同试剂在光照下进行反应时获得的模式进行比较。观察到链的标记方式存在差异。讨论了使用在黑暗和光照条件下可呈现两种几何形式的相同标记试剂来绘制抗体活性位点图谱的可能性。
