关于大鼠肝脏微粒体上NADPH-细胞色素c还原酶定位的免疫化学和免疫电子显微镜研究。
Immunochemical and immunoelectron microscope studies on localization of NADPH-cytochrome c reductase on rat liver microsomes.
作者信息
Morimoto T, Matsuura S, Sasaki S, Yashiro Y, Omura T
出版信息
J Cell Biol. 1976 Feb;68(2):189-201. doi: 10.1083/jcb.68.2.189.
Abstract
By the use of ferritin-conjugated antibody (conjugate) indirect immunoelectron microscopy, NADPH-cytochrome c reductase was localized on rat liver microsomes. Most microsomes in the sections had from 1 to 12 conjugates on their outer surfaces. Among the conjugates, 83% was estimated to bind to NADPH-cytochrome c reductase at a molecular ratio of 1:1, 12% at the ratio of 2:1, and 5% at the ratio of 3 or 4:1. The correlation between immunochemical and morphological data confirmed that most of the NADPH-cytochrome c reducatase reacted with the conjugates. Subsequent morphological analyses have revealed that the enzyme is distributed homogeneously on the outer surfaces of microsomes but heterogeneously within microsomes in groups of three to five enzyme molecules.
摘要
通过使用铁蛋白偶联抗体（偶联物）间接免疫电子显微镜技术，将NADPH - 细胞色素c还原酶定位在大鼠肝脏微粒体上。切片中的大多数微粒体在外表面有1至12个偶联物。在这些偶联物中，估计83%以1:1的分子比例与NADPH - 细胞色素c还原酶结合，12%以2:1的比例结合，5%以3或4:1的比例结合。免疫化学和形态学数据之间的相关性证实，大多数NADPH - 细胞色素c还原酶与偶联物发生反应。随后的形态学分析表明，该酶均匀分布在微粒体的外表面，但在微粒体内以三到五个酶分子的组为单位呈异质性分布。
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