A modified method for tissue localization of cells bearing a complement receptor.

A modification of the method originally described by Dukor et al. (1970) was used for localization of cells bearing a complement receptor in lymphoid tissue of man, guinea pig, mouse and rat. Optimal fixation of the adherent indicator erythrocytes to the tissue section and satisfactory morphology of the tissue were obtained with a mixture of 1% paraformaldehyde and 0.5% glutaraldehyde. In addition, selective staining of indicator erythrocyte hemoglobin with Amido Black resulted in high contrast with the tissue constituents. The combination of fixative and stain descirbed above permitted more precise localization of cells bearing complement receptors in tissue sections.