Kamp E M, ter Laak E A, de Jong M F
Department of Bacteriology, Central Veterinary Institute, Lelystad, The Netherlands.
Vet Rec. 1990 Apr 28;126(17):434-7.
This paper is the first report of the production of a dermonecrotic toxin by pasteurella strains that do not belong to the species Pasteurella multocida subspecies multocida. Four strains, isolated from cattle with atrophic rhinitis, were characterised phenotypically. The strains were related to pasteurellaceae, but their taxonomic position remained unclear. The strains produced a toxin that caused a haemorrhagic dermonecrosis in guinea pigs and was lethal to mice. Both effects were neutralised by an antiserum against the purified dermonecrotic toxin of P multocida subspecies multocida. Western blot analysis of culture filtrates of the bovine strains revealed a protein, with the same molecular weight as dermonecrotic toxin, which reacted with both polyclonal and monoclonal antibodies against the toxin. In an immunodiffusion test, anti-dermonecrotic toxin serum did not discriminate between the toxin of the bovine strains and the toxin of P multocida subspecies multocida. It is concluded that these atypical pasteurella strains produce a toxin that is closely related to the dermonecrotic toxin of P multocida subspecies multocida.
本文首次报道了不属于多杀巴斯德菌多杀亚种的巴斯德菌菌株产生皮肤坏死毒素的情况。从患有萎缩性鼻炎的牛身上分离出4株菌株，并对其进行了表型特征分析。这些菌株与巴斯德菌科有关，但其分类地位仍不明确。这些菌株产生的毒素可导致豚鼠出现出血性皮肤坏死，并对小鼠具有致死性。这两种效应均被针对多杀巴斯德菌多杀亚种纯化皮肤坏死毒素的抗血清中和。对牛源菌株培养滤液的蛋白质印迹分析显示，有一种蛋白质的分子量与皮肤坏死毒素相同，它能与针对该毒素的多克隆抗体和单克隆抗体发生反应。在免疫扩散试验中，抗皮肤坏死毒素血清无法区分牛源菌株的毒素和多杀巴斯德菌多杀亚种的毒素。得出的结论是，这些非典型巴斯德菌菌株产生的毒素与多杀巴斯德菌多杀亚种的皮肤坏死毒素密切相关。
