Tovar J A, Mahour G H, Miller S W, Isaacs H, Smith C N
J Pediatr Surg. 1976 Feb;11(1):23-30. doi: 10.1016/0022-3468(76)90165-2.
Healthy 6-8-wk-old New Zealand white rabbits were injected with chromium-chloride- or sodium-chromate-labeled E. coli endotoxin after rapid infusion (10 ml/kg in 1 hr) or slow and repeated infusions (40 ml/kg daily for 7 consecutive days) of 10% Intralipid. Endotoxin clearance rates and RES organ uptakes were determined and the results were compared with those of the controls treated with correspondingly equal volumes of 5% D/W instead of fat. In the acute experiment, the clearance rates were similar in all animals during the first 15 min following endotoxin injection. After this phase, however, experimental animals had faster endotoxin clearance and eventually higher organ uptakes than the controls. In the chronic experiment, there was no significant difference in endotoxin clearance rates or total and per-gram organ uptakes between experimental and corresponding control animals infused with 5% D/W instead of fat. Experimental animals, particularly those having received multiple infusions of fat emulsion, showed deposition of polarizable brown pigment inside and outside the reticuloendothelial cells in the liver, spleen, and bone marrow. None of the controls had these pigments in their organs.
对6至8周龄的健康新西兰白兔，在快速输注（1小时内输注10毫升/千克）或缓慢重复输注（连续7天每天输注40毫升/千克）10%英脱利匹特后，注射氯化铬或铬酸钠标记的大肠杆菌内毒素。测定内毒素清除率和网状内皮系统（RES）器官摄取量，并将结果与用等量5%葡萄糖溶液而非脂肪处理的对照组进行比较。在急性实验中，内毒素注射后的前15分钟内，所有动物的清除率相似。然而，在此阶段之后，实验动物的内毒素清除速度更快，最终器官摄取量也高于对照组。在慢性实验中，输注5%葡萄糖溶液而非脂肪的实验动物与相应对照组动物在内毒素清除率、器官总摄取量和每克器官摄取量方面无显著差异。实验动物，尤其是多次输注脂肪乳剂的动物，在肝脏、脾脏和骨髓的网状内皮细胞内外出现了可极化的棕色色素沉积。对照组动物的器官中均未出现这些色素。
