Castle L A, Meinke D W
Department of Botany, Oklahoma State University, Stillwater 74078.
Plant Cell. 1994 Jan;6(1):25-41. doi: 10.1105/tpc.6.1.25.
Arabidopsis fusca mutants display striking purple coloration due to anthocyanin accumulation in their cotyledons. We describe six recessive fusca mutants isolated from Agrobacterium-transformed Arabidopsis families. These mutants first become defective during embryogenesis and exhibit limited seedling development. Double mutant constructs revealed that developmental defects were not simply a consequence of anthocyanin accumulation. fusca seedlings showed altered responses to several environmental and endogenous factors. Allelism tests established that three fusca loci are represented by mutants previously described as defective in light-regulated responses. To study the molecular basis of the fusca phenotype, we cloned the FUS6 gene. FUS6 encodes a novel protein that is hydrophilic, alpha-helical, and contains potential protein kinase C phosphorylation sites. The FUSCA proteins appear to act in a network of signal transduction pathways critical for plant development.
拟南芥fusca突变体由于其子叶中花青素的积累而呈现出显著的紫色。我们描述了从农杆菌转化的拟南芥家族中分离出的六个隐性fusca突变体。这些突变体在胚胎发生过程中首先出现缺陷，并表现出有限的幼苗发育。双突变体构建表明，发育缺陷并非仅仅是花青素积累的结果。fusca幼苗对几种环境和内源因素的反应发生了改变。等位性测试确定，三个fusca位点由先前描述为光调节反应缺陷的突变体代表。为了研究fusca表型的分子基础，我们克隆了FUS6基因。FUS6编码一种新型蛋白质，该蛋白质具有亲水性、α螺旋结构，并含有潜在的蛋白激酶C磷酸化位点。FUSCA蛋白似乎在对植物发育至关重要的信号转导途径网络中发挥作用。
